Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors
Mohammad Sahajadul Alam,
M. Mizanur Rahman,
Md. Monowar Hossain,
Mohammed Abu Kawsar Sarker,
Md. Monzurul Islam,
Rokeya Begum,
Most Umme Habiba,
Gazi Nurun Nahar Sultana
Issue:
Volume 3, Issue 4, August 2018
Pages:
49-54
Received:
24 August 2018
Accepted:
14 September 2018
Published:
17 October 2018
Abstract: Like all cancers, Gastric cancer (GC) is also known to be of genetic origin. Genes are mutated in every steps of cell division, among them hMLH1 is a DNA mismatch gene which plays important role during carcinogenesis. This study was chalked out to find out the status of mutated hMLH1 Gene and its clinicopathological relevance among Bangladeshi gastric cancer patients. It was a cross sectional study conducted during January 2015 to April 2016. Tissue extracted from gastrectomy specimen of carcinoma stomach patients sent to specified laboratory. In the laboratory after DNA extraction, PCR, sequencing and analysis was done. In this study out of 19, exons 7 and 8 and introns 8 and 9 were studied after primer designing for hMLH1 gene mutation. After analysis, mutated gene were matched with clinicopathological parameters like age, sex, location of tumour, types and grade of tumour to see their relevance. Out of 45 patients, mutation was found in 15 patients (33.3%). Most gene alteration was found in elderly patients, more in male. Antral growth had more (80.0%) mutation and in ulceroproliferative type (66.7%) (p < .005). Mutation was common in intestinal type (73.3%) of GC. hMLH1 gene mutation found more in the moderately differentiated carcinoma patients. Factors like age, sex, morphology, Lauren’s type, grading, personal habits etc. might play pivotal role in the development of gene mutation. Multi-centre large study are required to extract more relevant information in this regard.
Abstract: Like all cancers, Gastric cancer (GC) is also known to be of genetic origin. Genes are mutated in every steps of cell division, among them hMLH1 is a DNA mismatch gene which plays important role during carcinogenesis. This study was chalked out to find out the status of mutated hMLH1 Gene and its clinicopathological relevance among Bangladeshi gast...
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Cervical Smears: Morphological and Epidemiological Study for Intra-Epithelial Neoplasia in Lubumbashi
Mwenze Didier,
Mulenga Phillipe,
Mukalay Abdon,
Ilunga Julien
Issue:
Volume 3, Issue 4, August 2018
Pages:
55-58
Received:
1 September 2018
Accepted:
29 September 2018
Published:
25 October 2018
Abstract: Cervical carcinoma is one of the most preventable cancers for conventional cervical smear may detect intra-epithelial neoplasia before the invasive stage. Often cervical intra-epithelial neoplasia may be detected by conventional cervical smear. There is no cancer control policy in the Democratic Republic of Congo (DRC), as there is no cancer registry. This study was done to determine the morphological and epidemiological profile of cervical pathology in Lubumbashi. Data were selected from the Congolese League against Cancer (LCCC) registry who organized in the Democratic Republic of Congo the first cervical voluntary screening in March 2012. In total, 639 women aged from 22 to 65 years underwent cervical smears. Histopathological examination was done in case of high grade intra-epithelial neoplasia. Among all the women, 30 were living with intra-epithelial neoplasia, 24.4 and 75.6% had high grade and low grade lesions. Cytological and histological correlation was found in 65% of high grade lesion. In conclusion, this study shows a significant frequency of intra-epithelial cervical neoplasia in Lubumbashi city. Women beyond 30 years present 11 times the risk of high grade intra-epithelial lesion compared to youngers. Data are in many cases not reproducible compared with some publications. This study calls for multicentrically evaluation of intra-epithelial cervical neoplasma and for cervical cancer.
Abstract: Cervical carcinoma is one of the most preventable cancers for conventional cervical smear may detect intra-epithelial neoplasia before the invasive stage. Often cervical intra-epithelial neoplasia may be detected by conventional cervical smear. There is no cancer control policy in the Democratic Republic of Congo (DRC), as there is no cancer regist...
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