Colorectal cancer affects a large number of people. There is an urgent need to develop novel treatments for this disease. In recent years, research on medical applications of atmospheric low temperature plasma (ALTP) has been actively conducted, and cancer treatment is one of the targets. In this study, we focused on colorectal cancer and evaluated plasma as a new treatment method. In vivo and in vitro experiments were conducted using plasma-treated saline (PTS) and plasma-treated medium (PTM) prepared by submerged bubbling treatment using ALTP. In vivo experiments using a rat model of colorectal cancer revealed that PTS administration to the colon slowed tumor progression based on endoscopic and histopathological observations. To investigate the cause of the inhibition of tumor progression, we evaluated the impact of ALTP on colon cancer cells. As a result of culturing colorectal cancer cells with PTM, cell proliferation was inhibited. Also, cell death was induced by cell swelling. qPCR revealed that PTMs induced cell death of cancer cells through signaling of tumor necrosis factor α (TNF-α), an inflammatory cytokine. Therefore, the inhibition of cancer progression by PTS in the rat model occurs by inducing cell death through the dissolution of ALTP components in the liquid.
| Published in | International Journal of Clinical Oncology and Cancer Research (Volume 7, Issue 1) |
| DOI | 10.11648/j.ijcocr.20220701.12 |
| Page(s) | 8-13 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Adenocarcinoma, Tumor Necrosis Factor-alpha, Therapeutics, Reactive Oxygen Species, Atmospheric Low Temperature Plasma
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APA Style
Genu Takahashi, Nanako Okuno, Kyota Yoshino, Takamichi Hirata, Chihiro Kobayashi, et al. (2022). The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205. International Journal of Clinical Oncology and Cancer Research, 7(1), 8-13. https://doi.org/10.11648/j.ijcocr.20220701.12
ACS Style
Genu Takahashi; Nanako Okuno; Kyota Yoshino; Takamichi Hirata; Chihiro Kobayashi, et al. The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205. Int. J. Clin. Oncol. Cancer Res. 2022, 7(1), 8-13. doi: 10.11648/j.ijcocr.20220701.12
AMA Style
Genu Takahashi, Nanako Okuno, Kyota Yoshino, Takamichi Hirata, Chihiro Kobayashi, et al. The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205. Int J Clin Oncol Cancer Res. 2022;7(1):8-13. doi: 10.11648/j.ijcocr.20220701.12
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Download@article{10.11648/j.ijcocr.20220701.12,
author = {Genu Takahashi and Nanako Okuno and Kyota Yoshino and Takamichi Hirata and Chihiro Kobayashi and Akira Mori and Masaya Watada},
title = {The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205},
journal = {International Journal of Clinical Oncology and Cancer Research},
volume = {7},
number = {1},
pages = {8-13},
doi = {10.11648/j.ijcocr.20220701.12},
url = {https://doi.org/10.11648/j.ijcocr.20220701.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20220701.12},
abstract = {Colorectal cancer affects a large number of people. There is an urgent need to develop novel treatments for this disease. In recent years, research on medical applications of atmospheric low temperature plasma (ALTP) has been actively conducted, and cancer treatment is one of the targets. In this study, we focused on colorectal cancer and evaluated plasma as a new treatment method. In vivo and in vitro experiments were conducted using plasma-treated saline (PTS) and plasma-treated medium (PTM) prepared by submerged bubbling treatment using ALTP. In vivo experiments using a rat model of colorectal cancer revealed that PTS administration to the colon slowed tumor progression based on endoscopic and histopathological observations. To investigate the cause of the inhibition of tumor progression, we evaluated the impact of ALTP on colon cancer cells. As a result of culturing colorectal cancer cells with PTM, cell proliferation was inhibited. Also, cell death was induced by cell swelling. qPCR revealed that PTMs induced cell death of cancer cells through signaling of tumor necrosis factor α (TNF-α), an inflammatory cytokine. Therefore, the inhibition of cancer progression by PTS in the rat model occurs by inducing cell death through the dissolution of ALTP components in the liquid.},
year = {2022}
}
TY - JOUR T1 - The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205 AU - Genu Takahashi AU - Nanako Okuno AU - Kyota Yoshino AU - Takamichi Hirata AU - Chihiro Kobayashi AU - Akira Mori AU - Masaya Watada Y1 - 2022/02/09 PY - 2022 N1 - https://doi.org/10.11648/j.ijcocr.20220701.12 DO - 10.11648/j.ijcocr.20220701.12 T2 - International Journal of Clinical Oncology and Cancer Research JF - International Journal of Clinical Oncology and Cancer Research JO - International Journal of Clinical Oncology and Cancer Research SP - 8 EP - 13 PB - Science Publishing Group SN - 2578-9511 UR - https://doi.org/10.11648/j.ijcocr.20220701.12 AB - Colorectal cancer affects a large number of people. There is an urgent need to develop novel treatments for this disease. In recent years, research on medical applications of atmospheric low temperature plasma (ALTP) has been actively conducted, and cancer treatment is one of the targets. In this study, we focused on colorectal cancer and evaluated plasma as a new treatment method. In vivo and in vitro experiments were conducted using plasma-treated saline (PTS) and plasma-treated medium (PTM) prepared by submerged bubbling treatment using ALTP. In vivo experiments using a rat model of colorectal cancer revealed that PTS administration to the colon slowed tumor progression based on endoscopic and histopathological observations. To investigate the cause of the inhibition of tumor progression, we evaluated the impact of ALTP on colon cancer cells. As a result of culturing colorectal cancer cells with PTM, cell proliferation was inhibited. Also, cell death was induced by cell swelling. qPCR revealed that PTMs induced cell death of cancer cells through signaling of tumor necrosis factor α (TNF-α), an inflammatory cytokine. Therefore, the inhibition of cancer progression by PTS in the rat model occurs by inducing cell death through the dissolution of ALTP components in the liquid. VL - 7 IS - 1 ER -
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