Volume 3, Issue 4, August 2018, Page: 49-54
Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors
Mohammad Sahajadul Alam, Department of Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh
M. Mizanur Rahman, Department of Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh
Md. Monowar Hossain, Department of Surgical Oncology, Khulna Medical College, Khulna, Bangladesh
Mohammed Abu Kawsar Sarker, Department of Surgery, Aichi Medical College, Dhaka, Bangladesh
Md. Monzurul Islam, Department of Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh
Rokeya Begum, Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh
Most Umme Habiba, Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh
Gazi Nurun Nahar Sultana, Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh
Received: Aug. 24, 2018;       Accepted: Sep. 14, 2018;       Published: Oct. 17, 2018
DOI: 10.11648/j.ijcocr.20180304.11      View  553      Downloads  19
Abstract
Like all cancers, Gastric cancer (GC) is also known to be of genetic origin. Genes are mutated in every steps of cell division, among them hMLH1 is a DNA mismatch gene which plays important role during carcinogenesis. This study was chalked out to find out the status of mutated hMLH1 Gene and its clinicopathological relevance among Bangladeshi gastric cancer patients. It was a cross sectional study conducted during January 2015 to April 2016. Tissue extracted from gastrectomy specimen of carcinoma stomach patients sent to specified laboratory. In the laboratory after DNA extraction, PCR, sequencing and analysis was done. In this study out of 19, exons 7 and 8 and introns 8 and 9 were studied after primer designing for hMLH1 gene mutation. After analysis, mutated gene were matched with clinicopathological parameters like age, sex, location of tumour, types and grade of tumour to see their relevance. Out of 45 patients, mutation was found in 15 patients (33.3%). Most gene alteration was found in elderly patients, more in male. Antral growth had more (80.0%) mutation and in ulceroproliferative type (66.7%) (p < .005). Mutation was common in intestinal type (73.3%) of GC. hMLH1 gene mutation found more in the moderately differentiated carcinoma patients. Factors like age, sex, morphology, Lauren’s type, grading, personal habits etc. might play pivotal role in the development of gene mutation. Multi-centre large study are required to extract more relevant information in this regard.
Keywords
Hmlh1 Gene Mutation, Gastric Cancer, Clinicopathological Relevance
To cite this article
Mohammad Sahajadul Alam, M. Mizanur Rahman, Md. Monowar Hossain, Mohammed Abu Kawsar Sarker, Md. Monzurul Islam, Rokeya Begum, Most Umme Habiba, Gazi Nurun Nahar Sultana, Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors, International Journal of Clinical Oncology and Cancer Research. Vol. 3, No. 4, 2018, pp. 49-54. doi: 10.11648/j.ijcocr.20180304.11
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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